Draft genome sequence of the pulse crop blackgram [Vigna mungo (L.) Hepper] reveals potential R-genes

نویسندگان

چکیده

Abstract Blackgram [ Vigna mungo (L.) Hepper] (2n = 2x 22), an important Asiatic legume crop, is a major source of dietary protein for the predominantly vegetarian population. Here we construct draft genome sequence blackgram, first time, by employing hybrid assembly with Illumina reads and third generation Oxford Nanopore sequencing technology. The final de novo whole blackgram ~ 475 Mb (82% genome) has maximum scaffold length 6.3 N50 1.42 Mb. Genome analysis identified 42,115 genes mean coding 1131 bp. Around 80.6% predicted were annotated. Nearly half assembled composed repetitive elements retrotransposons as (47.3% transposable elements, whereas, DNA transposons made up only 2.29% genome. A total 166,014 SSRs, including 65,180 compound primer pairs 34,816 SSRs designed. Out 33,959 proteins, 1659 proteins showed presence R-gene related domains. KIN class was found in majority (905) followed RLK (239) RLP (188). will facilitate identification agronomically accelerate genetic improvement blackgram.

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ژورنال

عنوان ژورنال: Scientific Reports

سال: 2021

ISSN: ['2045-2322']

DOI: https://doi.org/10.1038/s41598-021-90683-9